Table 3 Data extraction instrument
General information about the study | Â |
|---|---|
Authors | Zizhi Tang Jun Chen, Ming Zeng, Xiaojun Wang, Chang Guo, Peng Yue, Xiaohu Zhang, Huiqiang Lou, Dezhi Mu, Daochun Kong, Antony M. Carr, and Cong Liu |
Year of publication | 23rd May 2022 |
Country in which it was conducted | China and UK |
Details of the study | |
Aim | To characterize the potential role of ENDOD1 in DNA repair |
Context | This study uncovers the role of ENDOD1 in the context of DNA repair pathway and its synthetically lethal interaction with TP53 which is a well-known tumor suppressor gene. It also explores the possibility of ENDOD1 serving as a specific target against cancer cells in view of its synthetic lethal relationship with TP53 |
Type of study | In vitro study |
Method | Cell proliferation and viability; Clonogenic assays: Comet Assay to assess DNA repair efficiency; CCK8 colorimetry to show the siRNA knock out efficiency; Measurement for PARP-DNA complex; Immunofluorescence Immunofluorescentstaining and Western Blotting; Flow cytometry and fluorescent-activated cell sorting (FACS); Animal, biopsies and histochemical staining |
Key findings | Identified ENDOD1 as a potential wide-spectrum and cancer-specific target for SL drug discovery |